My research focuses on understanding the cell biological mechanisms driving cell behaviors common to morphogenesis and cancer progression, and how they are coordinated by developmental patterning. I use C. elegans as a model to leverage its strengths in live-cell imaging and genetic manipulation. Click on the headers below to learn more about my pre- and postdoctoral research:
I'm currently a postdoctoral research fellow in Dr. Bob Goldstein's lab at the University of North Carolina at Chapel Hill. I'm utilizing my background in developmental biology to understand how cell biological mechanisms are regulated. I co-led a project characterizing the organization of the actomyosin network driving apical constriction during C. elegans gastrulation [Zhang, Medwig-Kinney & Goldstein, 2023].
My current project focuses on understanding how cell shape changes are coordinated by cell fate and polarity cues. Stay tuned!
I conducted my dissertation research in Dr. David Matus's lab at Stony Brook University, investigating how invasive cell fate acquisition, differentiation, and maintenance are regulated. During C. elegans somatic gonad development, a specialized uterine cell called the anchor cell (AC) invades the underlying basement membrane utilizing cellular programs that are largely conserved across other contexts of cell invasion [Medwig-Kinney & Matus, 2017].
The AC is specified through a Notch-mediated cell fate decision between two initially equipotent cells, giving rise to the invasive post-mitotic AC and a proliferative ventral uterine (VU) cell. I collaborated with the Pani lab to develop tools [Medwig-Kinney et al., 2022, Pani et al., 2022] to visualize and perturb Notch/Delta signaling.
First, I showed that four pro-invasive transcription factors, FOS-1 (Fos), EGL-43 (EVI/MEL), HLH-2 (E/Daughterless), and NHR-67 (NR2E1/TLX) form a gene regulatory network with two sub-circuits: one controlling G0/G1 cell cycle arrest and another controlling cell cycle independent pro-invasive targets. The regulatory interactions among these transcription factors are dynamic, as these transcription factors function independently to regulate the Notch-mediated AC/VU cell fate decision [Medwig-Kinney & Smith et al., 2020]. Later work I contributed to demonstrated how chromatin modifiers regulate this network [Medwig-Kinney & Palmisano et al., 2021, Smith et al., 2022].
Tools and methods development:
During my time in lab, I also contributed to several collaborative tool-building efforts, including constructing a CDK biosensor to visualize cell cycle state live [Adikes, Kohrman & Martinez et al., 2020], adapting the NanoDam system for tissue-specific transcription factor profiling [Yee et al., preprint], expanding tools for and improving specificity of the auxin-inducible degron protein degradation system [Martinez et al., 2020; Ashley et al., 2021; Hills-Muckey et al., 2022], as well as optimizing techniques for microinjection [Gibney et al., 2023] and live-cell imaging using lattice light sheet microscopy [Liu et al., 2018].
I'm grateful to the following organizations that have provided funding to support my research: